Shared Instrument Facility

About

Location

Biodesign Shared Instrument
Biodesign Institute at ASU
1001 S McAllister Ave, Tempe, AZ 85281
Room A335A, Enter through A335A or B329

Overview of rules

• All Shared Instrument Facilities are free of charge to Biodesign faculty and staff
• All equipment is self-serve
• All instruments are used on a first-come, first-serve basis
• Access and training for all instruments is coordinated and supported by BEARS
• Instrumental maintenance and calibration performed by BEARS
• Lab courtesy rules apply
• ASU gas safety and instrument-specific training required for Jasco J-815 Spectropolariometer
• Instrument-specific training required for Nanosight NS300

Overview of tools

Jasco J-815 Circular Dichroism Spectropolarimeter

Measures and quantifies differences in absorption of left and right circularly polarized light in chiral molecules and macromolecular structures.

• Analyze secondary/tertiary structure or conformation of macromolecules
• Observe how secondary structure of proteins changes with environmental conditions or on interaction with other molecules
• Derive structural, kinetic and thermodynamic information
• ASU gas handling and instrument specific training required
• Researchers will provide their own cells / cuvettes; recommendations available based on application

Spectramax M5 plate reader (96/384 format)

Multimode photometrics for endpoint or kinetic measurements

• Absorbance (UV – VIS)
• Fluorescence
• Glow luminescence
• Time-resolved fluorescence
• Fluorescent polarization

ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated

Fluorescence based monitoring of PCR in real time to quantify nucleic acid transcripts.

• Gene expression assays / Genotyping analysis
• High resolution melt analysis
• miRNA and iRNA quantification
• Single cell PCR
• TaqMan Array Card ready

Biorad C1000 Thermal Cycler

Two module tube strip (48 tubes per module) multi-use programmable thermal cycler

• Two module tube strip (48 tubes per module) multi-use programmable thermal cycler
• Endpoint PCR
• Cloning reactions
• Provided as an overflow instrument for labs needing extra thermal cycler space
• Modules are independently programmable

Amersham ImageQuant 800

Basic CCD camera based blot / gel imaging

• Basic CCD Camera based blot / gel imaging
• General purpose gel and blot imaging
• Chemiluminescence Modes
• No Ethidium bromide gels — Safe stains only.

NanoDrop 8000 Spectrophotometer

Simple, hand-pipetted pedestal based spectrophotometer

• 8 channel model – 1uL required 2uL recommended
• Rapid DNA/RNA/Protein quantification
• Purity indicators A260/280 and A260/230 ratios
• Preconfigured methods for BCA, Bradford, Pierce 660 and Lowry assays

NanoSight NS300 Particle Characterization Platform

NTA (Brownian motion tracking) based size characterization and quantitation of particles

• 10 – 2000nm size range
• 10⁶ – 10⁹ particles / mL
• Viral vaccine and virus characterization
• Phage assembly
• Biomarker detection
• Protein aggregation
• Extracellular vesicle characterization

Hosted equipment

Hosted items are owned or supported by facilities but located within individual biodesign centers that have a complimentary research focus and large user base. All hosted items are available upon request and training completion, if applicable.

If you would like access to hosted equipment, to host a piece of equipment, or to offer an existing piece of equipment as a hosted item please contact Kyle Lewis.

• BioRad GelDoc EZ gel documentation system + All Accessories & trays
• Qsonica Q700 Sonicator; bath and probe available
• Agilent 2100 Bioanalyzer
• Typhoon Trio fluorescent gel scanner
• Large capacity shaking incubator
• Beckman Coulter ultra-centrifuges – Type 100ti, 45ti, and 50.2ti rotors available
• L100XP (3rd floor Biodesign B)
• L90K (3rd floor Biodesign A)
• L100XP (1st floor Biodesign A)